Related Experiment Videos
LONP2 Enhances Cervical Cancer Growth and Metastatic Potential by Upregulating PLS3 and Activating PI3K/AKT Signaling
Ying Yang1, Jing Sun1, Liu Tian1
1Department of Gynaecology and Obstetrics, The Third People's Hospital of the Xinjiang Uygur Autonomous Region, Urumqi, People's Republic of China.
Purpose:
This study aimed to investigate the role of LONP2 in cervical cancer progression and elucidate the underlying signaling mechanisms.
Methods:
HeLa cervical cancer cells were transduced with lentiviral vectors to overexpress LONP2 or simultaneously overexpress LONP2 and knock down plastin 3 (PLS3). Protein expression was assessed by Western blotting. Cell viability, apoptosis, migration, and invasion were evaluated using CCK-8 assays, flow cytometry, and Transwell assays, respectively. The involvement of the PI3K/AKT signaling pathway was examined by detecting the phosphorylation levels of PI3K and AKT. A nude mouse xenograft model was established to validate the in vitro findings.
Results:
LONP2 overexpression significantly increased PLS3 protein expression in cervical cancer cells. Functionally, LONP2 promoted cell proliferation, migration, invasion, and tumor growth while suppressing apoptosis. Knockdown of PLS3 markedly attenuated these tumor-promoting effects both in vitro and in vivo. Mechanistically, LONP2 overexpression enhanced the phosphorylation of PI3K and AKT without significantly affecting total PI3K or AKT expression, indicating activation of the PI3K/AKT signaling pathway. Importantly, silencing PLS3 effectively reversed LONP2-induced PI3K/AKT pathway activation. Consistent findings were observed in xenograft tumors, where PLS3 knockdown suppressed tumor growth and reduced p-PI3K and p-AKT expression.
Conclusion:
LONP2 promotes cervical cancer progression by upregulating PLS3 expression and activating the PI3K/AKT signaling pathway. The LONP2/PLS3/PI3K-AKT signaling axis represents a novel mechanism driving cervical cancer progression and may serve as a potential therapeutic target for cervical cancer treatment.
Related Concept Videos
Abnormal Proliferation
PI3K/mTOR/AKT Signaling Pathway
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
lncRNA - Long Non-coding RNAs