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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
The genome sequence of Petasites hybridus (L. G.Gaertn., B.Mey. & Scherb., 1802) (Asterales: Asteraceae)
Markus Ruhsam1, Peter M Hollingsworth1,
1Royal Botanic Garden Edinburgh, Edinburgh, Scotland, UK.
Abstract:
We present a genome assembly of Petasites hybridus (Butterbur; Streptophyta; Magnoliopsida; Asterales; Asteraceae). The assembly consists of two haplotypes with total lengths of 2 802.87 megabases and 2 756.82 megabases. Most of haplotype 1 (95.61%) is scaffolded into 30 chromosomal pseudomolecules. Haplotype 2 was assembled to scaffold level. The mitochondrial sequence has a length of 302.34 kilobases and the plastid genome assembly has a length of 150.22 kilobases. Gene annotation of this assembly on Ensembl identified 41 187 protein-coding genes. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.
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Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
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