Protocol for repeated Plasmodium chabaudi infection in a murine malaria model

In malaria-endemic regions, individuals experience repeated malaria infection throughout life. Here, we present a protocol to investigate the immunological mechanisms triggered by repeated exposure using a murine model of sequential infections with the rodent parasite Plasmodium chabaudi chabaudi. We provide a detailed and standardized series of steps covering primary infection, natural recovery without treatment, and subsequent secondary infection. This approach offers a robust framework to investigate the effects and long-term consequences of repeated malaria infections on the host. For complete details on use and execution of this protocol, please refer to Stephen et al.,¹ Pérez-Mazliah et al.,² and Achtman et al.³.