Related Experiment Video
Updated: Sep 23, 2026
Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
Published on: November 17, 2017
Protocol for spatially restricted CreERT2-mediated recombination in mouse brain-infiltrating B cells using
Mika Schneider1, Georgios Perdikaris1, Tobias Derfuss2
1Department of Biomedicine, University Hospital Basel and University of Basel, Basel, Switzerland; Multiple Sclerosis Centre and Research Center for Clinical Neuroimmunology and Neuroscience (RC2NB), University Hospital Basel and University of Basel, Basel, Switzerland.
Abstract:
Tamoxifen-dependent, cre-recombinase-mediated techniques enable precise temporal control of gene expression, but do not always provide sufficient anatomical control. Here, we present a protocol to restrict gene expression using local injection of 4-hydroxy-tamoxifen in bone wax. As an example, we describe the induction of the viral protein LMP1 and the fluorescent protein tdTomato only in brain-infiltrating B cells. We describe steps for intracerebral virus injection and 4-hydroxy-tamoxifen administration in mice. For complete details on the use and execution of this protocol, please refer to Kim et al.1.
