Related Experiment Video
Updated: Sep 23, 2026

Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions
Published on: January 5, 2024
The protein epiphany
1Department of Chemistry, University of York, Heslington, York, United Kingdom.
Abstract:
George Sheldrick made his reputation by providing excellent software to solve the structures of a range of crystals which diffracted well and had fewer than 200 atoms. The techniques used included Patterson searches, direct-methods phase determination and least-squares refinement of the final model. By the 1990s it was possible to collect atomic resolution data from macromolecules, so he adapted the least-squares refinement program to handle the large numbers of parameters required for these cases. Experimental phases for large molecules could be calculated based on the anomalous signal from heavy atoms such as sulfur. The structure-solution software was adapted to find the positions of the anomalous scatterers using the small anomalous differences, and then extended to first generate and refine the phases, and then interpret the resultant maps.
Related Concept Videos
Post-translational Translocation of Proteins to the RER
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...
Directing Proteins to the Rough Endoplasmic Reticulum
The Unfolded Protein Response
Tagging and Fusion Proteins
Bacterial Protein Maturation
Protein Organization

