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The quantitative and qualitative impairment of wound healing by adriamycin
Abstract:
Clinical impression suggests that Adriamycin (ADR) interferes with wound healing. To examine the effects of ADR on wound healing, male Fischer rats were subjected to a dorsal, midline, full-thickness longitudinal incision (day 0). Wound clips were removed on day +7. Twenty animals per group were given intravenous ADR on day -7, day 0, day +3 and day +7. Twenty animals served as non-treated, wounded controls (C). Five animals/group were sacrificed on days +7, +14 and +21, at which time two 9.5 mm wide strips were taken from each animal perpendicular to the wound axis and submitted for wound breaking strength (WBS) measurements and load-extension curve analysis. WBS differed most markedly at Day 21 between C(1671 +/- 59g) and ADR day -7(1360 + 71 g) p less than 0.01; C and ADR day 0 (1051 +/- 108 g) p less than 0.001; C and ADR day + 3(1134 +/- 176 g) p less than 0.02. No difference existed between C and day +7 (1790 +/- 153 g). A point of inflection always occurred between 55-60% elongation in ADR treated animals only. This portion of the curve has been previously shown to represent collagen content. It is concluded that perioperative ADR administration (day -7 through day +3) significantly and substantially impairs skin wound healing in the rat. A form of collagen yielding underlies and may contribute to this defect.
Insights
Adriamycin (ADR) administration significantly impairs rat skin wound healing, particularly when given perioperatively. This chemotherapy agent appears to affect collagen content, leading to reduced wound strength.
Area of Science:
- Oncology
- Wound Healing Research
- Pharmacology
Background:
- Clinical observations suggest Adriamycin (ADR) negatively impacts wound healing.
- Understanding ADR's effects on tissue repair is crucial for patient management during chemotherapy.
Purpose of the Study:
- To investigate the impact of Adriamycin (ADR) on the process of skin wound healing in a rat model.
- To quantify the effects of ADR administration timing on wound breaking strength and tissue properties.
Main Methods:
- Male Fischer rats underwent standardized dorsal skin incisions.
- Groups received intravenous Adriamycin (ADR) at different time points relative to wounding (day -7, 0, +3, +7).
- Wound breaking strength (WBS) and load-extension curves were analyzed at days +7, +14, and +21 post-wounding.
Main Results:
- Perioperative ADR administration (days -7, 0, +3) significantly reduced wound breaking strength by day 21 compared to controls.
- A distinct point of inflection in load-extension curves, indicative of collagen content, was observed only in ADR-treated animals.
- ADR administration on day +7 did not significantly affect wound healing parameters.
Conclusions:
- Perioperative Adriamycin (ADR) administration substantially impairs skin wound healing in rats.
- The observed defect in wound healing is associated with altered collagen content, suggesting a collagen-yielding mechanism.