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Cloning and Protein Expression of CpEst in Komagataella phaffii

Ana Laura Reyes-Reyes1,2, David A Torres-Añorve3,4, Francisco Valero5

  • 1LIBBA Laboratory, Industrial Biotechnology Unit, Center for Research and Assistance in Technology and Design of the State of Jalisco, A.C. (CIATEJ), Guadalajara, Jalisco, Mexico. reyes.ana@inifap.gob.mx.

Methods in Molecular Biology (Clifton, N.J.)
|September 23, 2026
PubMed

Keywords:
CpEstEsteraseKomagataella phaffii

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Reproductive Cloning01:27

Reproductive Cloning

Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...