Related Experiment Video
Updated: Sep 26, 2026

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
Published on: May 4, 2018
Research Progress on Target Gene Screening and the Precise Control Potential of Diaphorina citri Using RNA
Liping Ye1, Zhenrui He2, Xiang Li1
1College of Life Sciences, Gannan Normal University, Ganzhou 341000, China.
Abstract:
Diaphorina citri Kuwayama is the primary natural vector of citrus Huanglongbing (HLB). Prolonged use of chemical pesticides leads to insect resistance, reduces control efficacy, and causes ecological pollution. RNA interference (RNAi) enables sequence-specific gene silencing, representing a promising precision strategy for managing D. citri. This review summarizes advances in RNAi-based control of D. citri, focusing on three groups of target gene screening strategies based on essential functional genes, behavioral regulation genes, and D. citri-Candidatus Liberibacter asiaticus (CLas) interaction genes. It also compares double-stranded RNA (dsRNA) delivery systems and highlights key bottlenecks, including poor stability, inefficient delivery, off-target effects, and resistance evolution. Future research should focus on optimizing target gene screening, developing efficient delivery systems, and enhancing ecological safety assessments. Integrating RNAi technology with various environmentally friendly management strategies will advance the sustainable control of D. citri and citrus HLB.
Related Concept Videos
Experimental RNAi
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

