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[On the possibility of determining the species of saliva stains using quantitative enzyme immunoassay]
A A Gusarov1,2, V L Sidorov3, O D Yagmurov3
1Russian Center of Forensic Medical Expertise, Moscow, Russia.
Objective:
Determination of the species identity of saliva samples dried on gauze based on total human IgG using quantitative enzyme immunoassay.
Material And Methods:
Saliva samples taken from 10 donors and dried on gauze. The samples were tested by quantitative ELISA using the IgG-total-ELISA-HEMA test kit.
Results:
The optical density and concentration of total IgG in whole saliva extracts were determined in 1:10 and 1:50 dilutions. The sensitivity limit of the method was established.
Conclusion:
The experimental data obtained indicate a reliable identification of the origin of the studied saliva samples on gauze from humans, both in undiluted extracts and in a 1:10 dilution. This variant of quantitative ELISA has absolute specificity, sufficient sensitivity and high productivity, which determines the prospect of its use to establish the species identity of saliva traces.
