Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The relationship between stem cell seeding efficiency and position in cell cycle.

F C Monette, J B DeMello

    Cell and Tissue Kinetics
    |March 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Some observations on the growth requirements of multipotent stem cells under defined culture conditions.

    Progress in clinical and biological research·1990
    Same author

    The role of interleukin-3 and heme in the induction of erythropoiesis.

    Annals of the New York Academy of Sciences·1989
    Same author

    Hemin acts synergistically with interleukin-3 to promote the growth of multipotent stem cells (CFU-GEMM) in "serum-free" cultures of normal murine bone marrow.

    Experimental hematology·1988
    Same author

    Growth of murine multipotent stem cells in a simple "serum-free" culture system: role of interleukin-3, erythropoietin, and hemin.

    Experimental hematology·1988
    Same author

    Molecularly characterized factors governing the growth of murine multipotent stem cells in serum-depleted marrow cultures.

    Advances in experimental medicine and biology·1988
    Same author

    Sensitivity of murine multipotential stem cell colony (CFU-GEMM) growth to interleukin-3, erythropoietin, and hemin.

    Experimental hematology·1987

    Colony-forming cells in cell cycle show reduced splenic seeding efficiency. This means the spleen colony assay may underestimate the true number of these critical cells.

    Area of Science:

    • Hematology
    • Cell Biology
    • Stem Cell Research

    Background:

    • The spleen colony assay is a standard method for quantifying hematopoietic stem and progenitor cells.
    • Understanding factors affecting cell seeding is crucial for accurate stem cell enumeration.

    Purpose of the Study:

    • To compare the splenic seeding efficiency of colony-forming cells in different preparations.
    • To determine if cell cycle status influences the accuracy of the spleen colony assay.

    Main Methods:

    • Comparison of colony-forming cell seeding efficiency from normal, regenerating, and velocity-sedimented preparations.
    • Analysis of both cycling and non-cycling cells.
    • Utilizing the spleen colony assay for enumeration.

    Related Experiment Videos

    Main Results:

    • Colony-forming cells actively in cell cycle demonstrated a 50% reduction in splenic seeding compared to non-cycling cells.
    • Normal marrow and sedimented non-cycling cells showed higher seeding efficiency.

    Conclusions:

    • The spleen colony assay can underestimate the total number of colony-forming cells.
    • The degree of underestimation is directly proportional to the number of cells in the cell cycle.