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Updated: Sep 27, 2026

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
Published on: March 22, 2016
A Historical Account of Retroviral Assembly and Budding
1Department of Microbiology and Immunology, Renaissance School of Medicine, Stony Brook University, Stony Brook, New York, USA;
Abstract:
This historical review, written in the year the first-in-class drug targeting the human immunodeficiency virus type 1 (HIV-1) capsid protein was approved by the Food and Drug Administration, focuses on a significant piece of insight provided in an editorial published in the journal AIDS in 1991 by retrovirus investigators John Wills and Rebecca Craven. Their conceptual framework of retroviral assembly and budding provided a foundation that has been strengthened and expanded over the years through studies engaging structural biology, molecular biology, biochemistry, cell biology, and developments in imaging technology. The collective validates a long-held consensus that elucidating capsid assembly could lead to innovative and successful drug discovery.
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