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Updated: Sep 27, 2026
Spatially Resolved, Integrated Single-Cell Multiomic Profiling of the Transcriptome and Epigenomic Targets in Frozen Tissue Sections
Published on: June 12, 2026
Photolabile oligonucleotides with topological light gradients enable spatially resolved single-cell transcriptomics
Robert A Piscopio1, Alex Chialastri2, Chieh Wang2
1Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA, USA.
Abstract:
We introduce scSTAMP-seq (single-cell spatial transcriptomic and multiomic profiling), a modular light-encoded barcoding strategy that preserves spatial information while leveraging standard single-cell platforms. scSTAMP-seq labels live and fixed cells with cholesterol-conjugated, photocleavable hashtag oligonucleotides, followed by patterned light exposure that 'stamps' spatial coordinates onto individual cells before single-cell multiome sequencing. Here we apply scSTAMP-seq to human embryoids showing how spatial organization and epigenetic states coregulate cellular programs in heterogeneous biological systems.
