Knowledge and HIV-Related Stigmatizing Attitudes Among Healthcare Workers: A Cross-Sectional Study

Background: Healthcare workers are central to the care of people living with HIV/AIDS (PLHIV), yet their own stigmatizing attitudes can undermine it. This cross-sectional study assessed HIV/AIDS knowledge and stigmatizing attitudes toward PLHIV among healthcare workers and the factors associated with stigma. Methods: All physicians and nurses employed at three public hospitals in Mersin, Turkey (N = 2230), were invited to participate; because participation was voluntary and anonymous, the analysed sample was self-selected and is best characterised as a convenience sample. Data were collected over 45 days using an anonymous, self-administered questionnaire hosted on an online survey platform, comprising a 10-item HIV/AIDS knowledge questionnaire adapted from a previously used instrument and the Health Care Provider HIV/AIDS Stigma Scale (HPASS; Turkish version), which measures stereotyping, discrimination, and prejudice. Associations were examined using group comparisons, correlations, and multivariable linear regression. Results: Among 460 participants (median age 35.0 years, IQR 31.0-44.0; 63.5% female; response rate 21.7%), knowledge assessment scores were not associated with any stigma subdimension; instead, older age, being married, and greater professional experience were associated with higher stereotyping and discrimination, with age and experience remaining significant after multivariable adjustment (p < 0.05). Notably, 81.1% felt they would need to inform other staff when encountering a PLHIV, which may raise confidentiality concerns; 67.4% believed HIV is mostly acquired through risky behaviours, and only 69.1% were comfortable working with an HIV-positive colleague. Conclusions: Stigmatizing attitudes were common and were associated with age and professional experience rather than with knowledge assessment scores. These findings generate the hypothesis that interventions addressing the attitudinal and professional-culture dimensions of stigma-particularly among more senior staff-may be more effective than information provision alone. Because the design was cross-sectional and the knowledge questionnaire had limited validity evidence, longitudinal and interventional studies are needed to test this hypothesis.