Related Experiment Video
Updated: Sep 27, 2026

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration
Published on: May 19, 2016
Systematic Analysis of MRTFB Phosphoproteomic Datasets Reveals Co-Regulated Kinase Networks and Co-Occurring
Jetna John1, Vaishnavi Gopalakrishnan1, Suhail Subair1
1Centre for Integrative Omics Data Science (CIODS), Yenepoya (Deemed to be) University, Mangalore 575018, Karnataka, India.
Abstract:
Background: Myocardin-related transcription factor B (MRTFB) acts as a transcriptional coactivator for serum response factor (SRF) and regulates actin cytoskeletal dynamics. Despite its biological significance, the phosphoregulatory network, upstream kinases, and interactors remain poorly understood. Methods: To explore this, we analyzed global phosphoproteomic datasets to identify predominant phosphosites. We categorized phosphosites in other proteins as positively or negatively co-regulated with the predominant phosphosites of MRTFB and also integrated predicted upstream kinases and interactors of MRTFB. Further, phosphosite conservation, co-occurrence patterns, and functional and pathway enrichment analyses of co-regulated proteins were also performed. Results: We identified S66 and S921 as predominant phosphosites in MRTFB, with the highest detection frequency across multiple experimental conditions. These phosphosites were found to be conserved within the MRTF family and across multiple species. From the predicted upstream kinases, MAPK1, MAPK3, MAST3, and CSNK1A1 were identified as predicted upstream kinases. Co-occurrence analysis revealed high positive co-occurrence between the predominant phosphosites, S66 and S921, suggesting similar functional roles. Functional enrichment analysis highlighted the involvement of co-regulated proteins in the actin cytoskeleton pathway. Conclusions: This study provides the detailed phosphoproteomic landscape of MRTFB, mapping its upstream kinases and co-regulated proteins involved in actin cytoskeleton regulation.
Related Concept Videos
PI3K/mTOR/AKT Signaling Pathway
Protein Kinases and Phosphatases
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
MAPK Signaling Cascades
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Generation of Straight or Branched Actin Filaments
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Microtubule Associated Proteins (MAPs)

