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Updated: Sep 27, 2026

Modeling Healthy and Dysbiotic Vaginal Microenvironments in a Human Vagina-on-a-Chip
Published on: February 16, 2024
Association Between Vaginal Microbiota and Female Infertility: A Systematic Review and Meta-Analysis
Hanyue Zheng1, Dengyan Qi1, Mengjie Zhao1
1Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
Abstract:
Vaginal dysbiosis may contribute to female infertility, but evidence on specific microbiota patterns remains inconsistent. This meta-analysis updates the epidemiological evidence linking vaginal dysbiosis to reduced female fertility, with a particular focus on low-Lactobacillus vaginal microbiota (LL-VMB), bacterial vaginosis (BV), intermediate microbiota, and Community State Type (CST) subtypes. A systematic search was performed in PubMed, Embase, Web of Science, Scopus, and the Cochrane Library from inception to 21 July 2025. We included observational studies that compared vaginal microbiota status with infertility outcomes in women of reproductive age. The primary exposure factors investigated were LL-VMB, BV, intermediate microbiota, and specific CST subtypes. Data were screened and extracted by two independent researchers. Thirty-one studies were included, with twenty-three eligible for meta-analysis. Evidence certainty was rated as very low. Compared with the control group, LL-VMB (OR = 2.16, 95% CI: 1.59-2.95) and BV (OR = 2.17, 95% CI: 1.54-3.06) were significantly associated with increased infertility risk. Intermediate microbiota showed no significant association. Exploratory network meta-analysis suggested potential variations in infertility risk among CST subtypes. Future large-scale, longitudinal, and multi-omics investigations must elucidate the mechanisms linking specific microbiota structures to reduced fertility.
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