Assembling an Expanded Metabolome for Camu-Camu (Myrciaria Dubia): Bioactive and Nutritional Compounds Relevant to...

Camu-camu (Myrciaria dubia) is an Amazonian shrub associated with riverine ecosystems and is recognized for its high vitamin C content and a broad range of nutritional and specialized metabolites. Previous studies have focused on specific metabolites or bioactive metabolites associated with health issues. Therefore, a comprehensive metabolomic survey using multiple analytical methods islacking. To address this gap in the scientific literature, we prepared a focused metabolomic inventory by integrating curated literature records with proton nuclear magnetic resonance (¹H NMR) and liquid chromatography-mass spectrometry (LC-MS) observations from berry materials, including pulp, skin/peel, and seeds. Ourstudy added 107 metabolite annotation records that were not in the literature. The integration of the literature-derived and ourdatasets yielded a combined inventory of 260 metabolites. Seven solvents were compared using ¹H NMR spectroscopy, and CH₃OH:CD₃OD (9:1, v/v) provided the broadest assigned coverage. LC-MS expanded the detection of lower-abundance and structurally diverse features, particularly phospholipids, quaternary ammonium compounds, organic acids, amino acid derivatives, and related metabolites. Compound-level and class-level assignments were distinguished, and interpretations were limited to the confidence supported by the available analytical evidence. The resulting inventory provides an expanded foundation for camu-camu quality evaluation, solvent selection, clinical trials, and targeted functional food research.