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Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Published on: December 29, 2015
XRN1-Resistant Elements Are Located in the 3'-Terminal Regions of Certain Genome Segments of Jingmenviruses
Egor V Okhezin1,2, Alexander G Litov1,3, Ivan S Kholodilov1
1Laboratory of Biology of Arboviruses, FSASI "Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS" (Institute of Poliomyelitis), 108819 Moscow, Russia.
Abstract:
Jingmenviruses have been identified over the past decade. During this period, significant advancements have been made in understanding their diversity, biology, and genomic functionality. Conventionally characterized orthoflaviviruses are distinguished by a specific class of subgenomic RNAs (sfRNAs) that contain exoribonuclease-resistant RNA (xrRNA) structures. These sfRNAs are the products of incomplete 5'-3' degradation of genomic RNA by the host exoribonuclease XRN1. Resistance to complete hydrolysis is mediated by highly conserved secondary structural motifs localized within the 3' untranslated region (UTR) of the genomic RNA. In classical orthoflaviviruses, xrRNAs play key roles in regulating viral replication, modulating the host immune response, and driving host cell adaptation. In the present study, we evaluated the exonuclease resistance of the 3' UTR genomic RNA segments of Alongshan and Yanggou viruses using in vitro assays. Our results demonstrate that 1 and 4 segments of Alongshan virus and 3 and 4 segments of Yanggou tick virus exhibit resistance to XRN1-mediated degradation.
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