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Updated: Sep 28, 2026

Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria
Published on: November 23, 2019
Cell Biology of Botrytis: Live-Cell Imaging Techniques and Protein Localization Protocols
H M Suraj1, André Fleissner2, Jan A L van Kan3
1Laboratory of Phytopathology, Wageningen University & Research, Wageningen, The Netherlands.
Abstract:
Botrytis cinerea is a widely studied model organism for plant-fungal interactions. This chapter provides simple and easy protocols for live-cell imaging techniques and subcellular protein localization studies in B. cinerea in vitro. Using a simple microscopy setup, we describe methods for imaging spore germlings and hyphae and for studying protein dynamics in response to the antifungal compound α-tomatine. The protocols highlight the application of fluorescence microscopy to visualize sterol distribution and protein localization patterns, enabling deeper insights into fungal cell biology.
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