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A guide to selecting high-performing antibodies for Prosaposin (UniProt ID: P07602) for use in western blot and
Vera Ruíz Moleón1, Sara González Bolívar Bolívar1, Riham Ayoubi1
1Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute,, McGill University, Montreal, Québec, H3A 2B4, Canada.
Abstract:
Prosaposin functions as a regulator of lysosomal enzymatic activity and as a neuroprotective player when secreted. Here we have characterized nine Prosaposin commercial antibodies for western blot and immunoprecipitation using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.
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