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The diagnostic value of miR-1-3p in atopic dermatitis and its correlation with inflammatory factors
Lun Pan1, Jianping He2, Peng Han3
1Department of Cosmetic Dermatology, Zigong Fourth People's Hospital, China.
Introduction And Objective:
MicroRNAs (miRNAs), such as miR-1-3p, play a crucial role in inflammatory processes, including atopic dermatitis (AD). The aim of the study is to investigate the role of miR-1-3p in AD, and its correlation with inflammation.
Material And Methods:
miR-1-3p level was analyzed using qRT-PCR. Its diagnostic value was assessed via ROC curve analysis. Correlations between miR-1-3p and inflammatory factors were assessed by Spearman's rank correlation coefficient. Proliferation of HaCaT cells were detected by CCK-8. The influence of miR-1-3p for inflammatory factors were examined through enzyme-linked immunosorbent assay (ELISA). Target genes of miR-1-3p were predicted using bioinformatics databases.
Results:
miR-1-3p was upregulated in AD patients and had a high diagnostic value (area under ROC curve=0.927, sensitivity=0.830, specificity=0.934) for AD. miR-1-3p was negatively correlated with lymphocytes (r=-0.558), and positively correlated with CRP (r=0.570), IL-6 (r=0.511), and IL-22 (r=0.596) levels, respectively. A high miR-1-3p level in HaCaT cells suppressed proliferation and increased inflammatory cytokine levels. Bioinformatics analysis identified 61 ovrerlapping target genes of miR-1-3p, enriched in pathways associated with inflammation and immune response.
Conclusions:
miR-1-3p is elevated in AD and associated with inflammation, suggesting a role in AD pathogenesis. Its effects on HaCaT cells and correlation with inflammatory markers indicate a mechanistic involvement in AD.