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Updated: Oct 1, 2026
Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
Published on: April 3, 2017
Alcalase-hydrolyzed casein peptides after simulated gastrointestinal digestion alleviate high-fat diet-induced
Ling Mo1,2, Chaochao Zhao2, Tian Wang2
1Wuzhou Red Cross Hospital, Wuzhou, Guangxi, China.
Background And Aim:
High-fat diet (HFD)-induced metabolic dysfunction is associated with disturbances in gut microbial composition and hepatic lipid metabolism. This study investigated the metabolic effects of Alcalase-hydrolyzed casein peptides after simulated gastrointestinal digestion (MCPs) in HFD-fed mice and explored their potential association with gut microbiota and fecal metabolic alterations.
Results:
MCP supplementation attenuated body weight gain, improved serum lipid profiles, and alleviated hepatic steatosis and injury in HFD-fed mice. In the colon, MCPs ameliorated histological damage and suppressed the mRNA and protein expression of pro-inflammatory cytokines, including IL-6, IL-1β, and TNF-α. Gut microbiota analysis revealed increased relative abundances of Akkermansia, Bifidobacterium, and Lactobacillus and reduced abundance of Desulfovibrio following MCP supplementation. Fecal metabolomics identified alterations in bile acid- and lipid metabolism-related signatures, including increased levels of tauroursodeoxycholic acid (TUDCA) and reduced arachidonic acid metabolism-related metabolites. In the liver, MCPs downregulated. SREBP-1c/ACC/GPAT1 lipogenic signaling and enhanced Nrf2/HO-1-associated antioxidant responses. Correlation analysis revealed associations between microbial-metabolic alterations and host metabolic phenotypes.
Conclusion:
Collectively, MCPs may alleviate HFD-induced metabolic dysfunction by modulating gut microbiota-associated metabolic profiles and improving intestinal and hepatic metabolic responses.
