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Updated: Oct 2, 2026
Methods for the Self-integration of Megamolecular Biopolymers on the Drying Air-LC Interface
Published on: April 7, 2017
Integration multidimensional assessment, bioactivities and chemometrics to study the effect of different drying
Ran Miao1, Heng Lu1, Hongjing Dong1
1Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
Abstract:
This study proposed an integration strategy basing on combination of multidimensional assessment, bioactivities and chemometrics to study the effect of different drying methods on Trichosanthis Fructus polysaccharides (TFPs). Polysaccharides content and molecular weight (Mw), monosaccharide composition, polysaccharides hydrolysates profiles and bioactivities (antioxidant and hypoglycemic activity) for TFPs treated with different drying methods were determined by phenol‑sulfuric acid method, high performance gel permeation chromatography-evaporative light scattering detector (HPGPC-ELSD), high-performance anion-exchange chromatography-pulsed amperometric detection (HPAEC-PAD), hydrophilic interaction chromatography-charged aerosol detection (HILIC-CAD-ESI-TOF/MS) and in vitro assays, respectively. Chemometrics analysis was employed for the classification and structure-activity relationship study of TFPs treated with different drying methods. The results indicated that the chemical characteristics and bioactivities of TFPs varied markedly after been treated with different drying methods. Freeze drying (FD) had the highest polysaccharide content (22.84 ± 0.120%) and the lowest Mw (Mw1: 8.31 ± 0.37 kDa, Mw2: 5.23 ± 0.004 kDa). The mole ratio of the monosaccharide composition for different drying methods varied obviously. Microwave drying (MD) (400 W) showed obvious Hydroxyl radical (•OH) scavenging activity, α-amylase and α-glucosidase inhibitory activities. TFPs hydrolysates profile basing HILIC-CAD combining with partial least squares discriminant analysis achieved the accurately distinguishing of Trichosanthis Fructus samples treated with different drying methods. Correlation analysis revealed the positive or negative correlation (P < 0.05) between some of the chemical composition and bioactivities of TFPs. Hierarchical clustering analysis preliminarily reflected the interactive variations and associations of different drying methods. This study provides methods and data to comprehensively assess drying effects on Trichosanthis Fructus, facilitating its industrial application.
