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Updated: Oct 3, 2026
Application of Live Mitochondria Staining in Cell-Sorting to Purify Hepatocytes Derived from Human Induced Pluripotent Stem Cells
Published on: December 1, 2023
Protocol for sorting hepatocyte-derived extracellular vesicles from mouse liver tissues using immunomagnetic beads
Cheng-Han Li1, Si-Qi Ying2, Qin-Lin Xu3
1State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, China; Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
Abstract:
Here, we present a protocol for isolating total liver tissue-derived extracellular vesicles (LT-EVs) from mouse liver tissue and purifying hepatocyte-derived extracellular vesicles (Hep-EVs) using asialoglycoprotein receptor (ASGPR)-based immunomagnetic sorting. We describe steps for mouse liver perfusion, enzymatic digestion, differential centrifugation, ASGPR labeling, and magnetic-activated cell sorting (MACS)-based enrichment. We then detail procedures for extracellular vesicle (EV) quality control by flow cytometry, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and western blotting. For complete details on the use and execution of this protocol, please refer to Ying et al.1.
