Stimuli-responsive vesicles in water-resistant nanofibers for molecular random access in DNA data storage

DNA is an attractive medium for data storage. Enabling efficient random access is essential for the practical use of DNA-based memory. We present a strategy for random access based on the selective release of target DNA from a nanofiber mesh matrix. Messages encoded in sets of DNA sequences are encapsulated inside stimuli-responsive small unilamellar vesicles (SUVs), embedded in an electrospun water-resistant nanofiber mesh. The target sequences are selectively released using orthogonal pH-based triggers. The orthogonality of these triggers enables the preservation of the SUVs containing the non-selected DNA, which remains in the fiber mesh for subsequent retrieval. This work combines sequence-independent chemical addressing with physical compartmentalization and double-layered protection. It represents initial steps towards a PCR-independent molecular selection system for random access in water-resistant fibers with potential applications in DNA-based memory architectures.