Related Experiment Video
Updated: Oct 7, 2026
Less-Invasive Technique for Non-stabilized Mandibular Fracture in Mouse Models
Published on: September 27, 2024
Protocol for standardized minimally invasive mouse models of bisphosphonate-related and radiation-induced jaw
Zhangfan Ding1, Jiayuan Zhang2, Hanyu Liu3
1State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Tissue and Tumor Microenvironments Lab, Cancer Discovery and Regenerative Medicine Program, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore.
Abstract:
We present a protocol for establishing murine models of bisphosphonate-related osteonecrosis of the jaw (BRONJ) and osteoradionecrosis of the jaw (ORNJ). We describe steps for a uniform, low-trauma tooth extraction technique with disease-specific induction, systemic zoledronic acid for BRONJ and precision-targeted irradiation for ORNJ, to generate robust and clinically relevant models of jaw osteonecrosis, approaching minimizes procedural variability and off-target effects, yielding robust, clinically relevant phenotypes. This protocol provides a standard and minimally invasive framework for investigating jaw osteonecrosis arising from distinct etiologies.