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Updated: Oct 7, 2026
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
Published on: November 30, 2016
OM-sqPCR enables one-step multiplex quantification of circulating small-RNA subclasses for colorectal cancer
Danping Guan1, Xiang Li2, Xiaoshan Zhang1
1Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China.
Abstract:
Accurate multiplex quantification of circulating small RNAs (sRNAs) enables comprehensive evaluation of their diagnostic potential. Here, we develop OM-sqPCR, a closed-tube, one-step multiplex RT-qPCR platform that simultaneously quantifies microRNAs, transfer RNA-derived sRNAs (tsRNAs), and ribosomal RNA-derived sRNAs (rsRNAs). By integrating optimized reaction chemistry for short RNA templates with computational primer-probe design, OM-sqPCR enables coordinated amplification of multiple sRNA subclasses in a single reaction. Applied to a multicenter cohort of 651 participants, OM-sqPCR identified a four-sRNA classifier that discriminated colorectal cancer from controls with an area under the ROC curve of 0.97, 85.9% sensitivity, and 93.1% specificity, with sustained performance in early-stage disease. The platform also supported measurement of previously reported sRNA panels for renal and thyroid cancer. These findings establish OM-sqPCR as a practical approach for multiplex circulating sRNA analysis and clinical biomarker evaluation.
