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Updated: Oct 8, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
Recent Progress in Understanding the Mechanism of Formate Dehydrogenases
1Department of Biochemistry, University of California, Riverside, California, USA.
Abstract:
Recent work concerning the reaction mechanism of the molybdenum-containing formate dehydrogenases is discussed, focusing on two alternate mechanisms of action for the enzyme involving either formation of CO2 or bicarbonate as the immediate product of the reaction. On the basis of the pH dependence of the rate of reduction of enzyme by formate and the results of a recent GC/MS study undertaken under single-turnover conditions indicating that no 18O from solvent H2 18O is incorporated into product CO2, it is concluded that bicarbonate cannot be formed in the course of the reaction, and that cysteine, once dissociated from the molybdenum, cannot be functioning as a base in the course of the reaction.
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