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Updated: Oct 10, 2026
Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
Published on: August 24, 2015
Protocol for establishing a murine Toscana virus infection model and downstream cellular analysis of skin tissue and
Yonca Keskek Turk1, Ailish McCafferty-Brown2, Clive S McKimmie3
1School of Medicine, University of Leeds, Leeds, UK; Virus Host Interaction Team, Skin Research Centre, York Biomedical Research Institute, Hull York Medical School, University of York, York, UK; Department of Medical Microbiology, Virology Unit, Faculty of Medicine, Hacettepe University, Ankara, Türkiye.
Abstract:
Here, we present an in vivo approach to study virus-vector-host interactions during Toscana virus (TOSV) infection in mouse skin. We describe preparation of viral stocks and subcutaneous TOSV inoculation into dorsal upper foot skin, either alone, with sand-fly salivary gland extract (SGE), or following sand-fly biting. We then detail tissue collection and generation of single-cell suspensions. Finally, we describe flow cytometric detection of infected cells, magnetic isolation of dermal fibroblasts, and RNA extraction for transcriptomic analysis. For complete details on the use and execution of this protocol, please refer to Keskek Turk et al.1.
