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Updated: Oct 11, 2026

Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes
Published on: March 8, 2013
Identifying Cardiac Autoantibody Biomarkers that Predict the Severity of Chagas Cardiomyopathy
Heriks Ribeiro1, Anton Dmitriev2, Antonia Pereira Rosa Neta3
1Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte, Natal, RN, Brazil; Department of Pediatrics, The Labatt Family Heart Centre and Translational Medicine, The Hospital for Sick Children, Toronto, Ontario, Canada.
Abstract:
Chagas cardiomyopathy (ChCM) affects up to 30% of patients with chronic Chagas disease and lacks reliable biomarkers for severity and conduction abnormalities. We profiled cardiac autoantibodies using 2-dimensional gel electrophoresis and mass spectrometry, followed by enzyme-linked immunosorbent assay validation and machine learning analysis. Six autoantibodies linked to glycolysis, adenosine triphosphate metabolism, and sarcomere structure distinguished ChCM stages (area under the curve: 0.87; 95% CI: 0.777-0.977) and a mean classification accuracy (0.84; 95% CI: 0.705-0.935). These findings suggest that an antibody-based panel may enable cost-effective risk stratification and prognostication in ChCM, with prospective validation before clinical application.
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