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Updated: Oct 11, 2026
Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
Published on: August 17, 2022
Preimplantation genetic testing laboratory classification strategies are associated with diagnostic yield and
Mina Popovic1, Erkan Kalafat2, Denny Sakkas3
1Department of Reproductive Medicine, Ghent University Hospital, Ghent, Belgium.
Objective:
To evaluate whether PGT-A laboratory was associated with diagnostic yield and live birth per single euploid embryo transfer.
Design:
Retrospective cohort study.
Subjects:
We analyzed a total of 9,630 PGT-A cycles performed between January 2015 and December 2023, comprising 40,308 blastocysts tested using trophectoderm biopsy and next generation sequencing. Three commercial PGT-A laboratories were utilized: Laboratory A (n = 18,480), Laboratory B (n = 19,261), and Laboratory C (n = 2,567). Clinical outcome analyses were restricted to 7,333 single euploid embryo transfers performed at the network's main clinic.
Exposure:
PGT-A laboratory.
Main Outcome Measures:
Euploidy rate, likelihood of having at least one euploid embryo available for transfer, and live birth rate per single euploid embryo transfer.
Results:
Overall, 52.3% (21,093/40,308) of blastocysts were diagnosed as euploid. Diagnostic yield differed between laboratories and varied according to maternal age. Compared with Laboratory A, Laboratory B had lower euploidy rates and a lower likelihood of obtaining at least one euploid embryo in younger patients, with higher euploidy rates observed among patients aged >42 years. Laboratory C had a consistently lower diagnostic yield in patients younger than 41 years. Among 7,333 single euploid embryo transfers performed at the main clinic, live birth rates per transfer were 49.8%, 41.6%, and 50.7% for Laboratories A, B, and C, respectively. After adjusting for maternal age, BMI, infertility indication, endometrial preparation and thickness, embryo grade, and biopsy day, Laboratory B remained associated with lower odds of live birth per single euploid embryo transfer compared to Laboratory A (OR=0.71, [95%CI: 0.64-0.79]; p<0.001), while Laboratory C was comparable (OR=1.08, [95%CI: 0.89-1.32]; p=0.418).
Conclusion:
PGT-A laboratory classification strategy was associated with both diagnostic yield and live birth per transfer. The discordance between these outcomes shows that euploidy rates alone do not provide a complete measure of laboratory performance and should be interpreted alongside downstream clinical outcomes.
