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Virus: mixed infection with herpes simplex and simian virus 40
Science (New York, N.Y.)
|March 25, 1966
Summary
Simian virus 40 (SV40) and herpes simplex virus can infect the same cell. This mixed infection occurs rarely with simultaneous inoculation but increases when herpes simplex virus follows SV40.
Area of Science:
- Virology
- Cell Biology
- Microbiology
Background:
- Mixed infection involves a single cell being infected by two distinct viruses.
- Understanding viral interactions is crucial for virology and disease research.
Purpose of the Study:
- To investigate the occurrence and conditions of mixed infection by simian virus 40 (SV40) and herpes simplex virus (HSV).
Main Methods:
- Electron microscopy was used to visualize viral presence in African green monkey kidney cell cultures.
- Sequential inoculation of SV40 and HSV was performed to assess infection dynamics.
Main Results:
- Mixed infection was observed in African green monkey kidney cells inoculated with SV40 and HSV.
- Simultaneous inoculation resulted in rare mixed infections.
- Inoculating HSV 24 hours after SV40 led to both viruses being present in approximately 5% of intact cell nuclei.
Conclusions:
- The timing of viral inoculation significantly impacts the rate of mixed infection.
- SV40 and HSV can co-infect the same cell nucleus under specific temporal conditions.