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MdAGL29 positively regulates MdBRC1/2 and negatively controls shoot branching in apple
Meng Ji1, Jin-Wei Li1, Fan Yang1
1Yangling Subsidiary Center of the National Apple Improvement Center, College of Horticulture, Northwest A & F University, Yangling, 712100, Shaanxi, China.
Key Message:
The MADS-box transcription factor MdAGL29 serves as a negative regulator of branching in apple by modulating MdBRC1/2 expression. Branch number is one of the major determinants in apple tree architecture. MdAGL29, a member of the MADS-box transcription factor family, is downregulated by 6-benzylaminopurine, a synthetic cytokinin. Heterologous overexpression of MdAGL29 in poplar resulted in fewer branches and lower endogenous cytokinin content compared with the control after decapitation treatment. Yeast one-hybrid and dual-luciferase reporter assays verified that MdAGL29 positively regulates the expression of branch negative regulators MdBRC1/2. Collectively, MdAGL29 acts as a negative regulator governing branch development in apple. These findings provide a promising target for precise regulation of plant branching.
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