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Bone marrow cytology as an adjunct tool for post-mortem interval estimation
Treasa James1, Akhilesh Pathak1, Ajay Kumar1
1Department of Forensic Medicine and Toxicology, All India Institute of Medical Sciences, Bathinda, India.
Introduction:
Estimating the post-mortem interval (PMI) remains challenging, particularly when external indicators are unreliable. Sternal bone marrow may provide a relatively stable substrate for PMI assessment.
Methods:
This observational autopsy-based study evaluated sternal bone marrow from 105 medico-legal autopsy cases with known PMI. Giemsa-stained aspirates were graded (1-5) for nuclear and cytoplasmic autolytic changes and grouped into five PMI intervals (<9 h to >27 h).
Results:
A highly significant association was observed between PMI and cellular degradation (p < 0.001). Early intervals showed preserved morphology, intermediate intervals showed nuclear budding and vacuolation, and advanced intervals demonstrated nuclear fragmentation, debris, and structural loss.
Discussion:
Sternal bone marrow cytology demonstrates a significant time-dependent association with PMI and may serve as a low-cost complementary tool during the early to intermediate post-mortem period. Further studies are required to establish its predictive accuracy and reproducibility.
