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Low-carbohydrate diets improved glucose homeostasis and physical performance by regulating mitochondrial function via
Tingting Li1,2, Xiaowen Wang1, Hao Lu1
1Shandong Second Medical University, School of Rehabilitation Medicine, Weifang 261053, Shandong, China.
Abstract:
This study aimed to analyze the effects of two low-carbohydrate diets (LCDs) on glucose homeostasis and physical performance in sedentary mice. Eighteen C57BL/6J mice were randomly divided into three groups: fed with standard chow (CON), high-fat LCD chow (HF-LCD), and high-protein LCD chow (HP-LCD). After two weeks, body weight (BW), food intake, fasting serum ketone bodies (β-OHB), blood glucose, glucose tolerance, and relative mass of skeletal muscle were measured. The physical performance was assessed using a treadmill endurance test, and the mRNA levels of mitochondrial function (SIRT3 and PGC1α) in the quadriceps were measured by qRT-PCR. Compared with the CON group, the HF-LCD group showed significantly increased fat content, β-OHB, and physical performance; the HP-LCD group showed significantly decreased calorie intake and BW. Compared with the HF-LCD group, the HP-LCD group showed significantly lower calorie intake, BW, fat content, β-OHB, skeletal muscle mass, and physical performance. Mechanistically, the mRNA levels of SIRT3-PGC1α in the HF-LCD group were higher than those in the other group. Both short-term LCDs can maintain glucose homeostasis in mice. However, only HF-LCD can increase β-OHB and physical performance without compromising skeletal muscle mass and strength. The underlying mechanism may involve transcriptional regulation of mitochondrial‑related genes via the SIRT3/PGC1α pathway.
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