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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
Published on: September 19, 2017
Interference-resistant palm electrochemical nickel-doped-enhanced cell-free biosensor for rapid multi-scenario
Jingjing Chen1, Haoran Wang2, Wei Pang2
1School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China; Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Military Medical Sciences Academy, Academy of Military Medical Sciences, Tianjin, 300050, China.
Abstract:
Composite nanomaterials-enabled USB electrochemical sensors hold great potential for on-site detection, yet achieving rapid, sensitive, and interference-free quantitative analysis across multiple scenarios through simple bioregistration modification remains to be explored. We developed an allosteric transcription factor-based nickel-doped anti-interference cell-free progesterone (ATF-Ni/AC-P4) sensor based on the structural transcription factor (SRTF), which enables efficient and simple modification of SRTF. The high affinity between SRTF and Ni@GO-MXene/SPEC provides exceptional sensitivity (0.0002 μmol L-1) and a wide detection range (0.001 μmol L-1 - 50 μmol L-1). ATF-Ni/AC-P4 sensor achieves one-step target recognition and ultra-fast signal output within 5 min across various sample matrices. Validated in matrices such as water, milk, urine, and blood, the system demonstrates excellent stability. The concurrently developed 3D-printed integrated membrane electrochemical device enables "sample-in, result-out" operation, requiring only simple pre-treatment of the sample. Compact, portable, and interference-resistant, the ATF-Ni/AC-P4 offers an innovative solution for food safety and on-site rapid detection.
