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Updated: Oct 17, 2026

Investigating the Alleviating Effects of Bacillus cereus Administration on Colitis through Gut Microbiota Modulation
Published on: July 27, 2022
Lactobacillus reuteri attenuates chronic diarrhea by modulating microbiota homeostasis and macrophage polarization
Xukai Cheng1, Yining Zhang1, Chao Li1
1Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China.
Abstract:
Chronic diarrhea (CD) severely affects patients' quality of life and is linked to ulcerative colitis, inflammatory bowel disease, and colorectal cancer. Gut microbiota dysbiosis plays a crucial role in intestinal health, with Lactobacillus reuteri emerging as a key regulator. However, its role in chronic diarrhea remains unclear. In this study, we observed macaques with spontaneous diarrhea and investigated microbial and metabolic changes. We also developed a chronic diarrhea mouse model using dextran sulfate sodium. CD group of both models revealed a significant reduction of L. reuteri. L. reuteri transplantation alleviates intestinal injury in CD by secreting D-lactate and modulates macrophage polarization. L. reuteri-derived D-lactate binds to IARS2 in intestinal epithelial macrophages, reducing mitochondrial IARS2 activation and suppressing cGAS-STING pathway activation. This preserves mitochondrial function while decreasing M1 macrophage polarization. Our research revealed the significance of microbiota dysbiosis in CD with both non-human primate and murine model, and highlighted the critical role of L. reuteri, which is conducted with D-lactate-mediated modulation of macrophage function and offered new insights into probiotic therapy for CD.
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