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Updated: Oct 17, 2026

Determining The Electromyographic Fatigue Threshold Following a Single Visit Exercise Test
Published on: July 27, 2015
Timing Matters: Assessing Fatigue During and After Resistance Training
Zhaoqian Li1, Penglin Diao2, Wenxin Chen1
1School of Physical Education, Shandong University, Jinan, China.
Background:
Monitoring neuromuscular fatigue during resistance training informs load prescription and recovery management. Two common approaches, namely within-training assessment (immediately after each set) and post-training assessment (after session completion), differ in their methodological demands, yet their acute responses and within-session reliability remain insufficiently characterized.
Hypothesis:
Fatigue levels would be influenced by training protocol, value type (maximum vs average), and set number or timepoint, with corresponding differences in within-session reliability.
Study Design:
Randomized, counterbalanced crossover study.
Level Of Evidence:
Level 2.
Methods:
A total of 13 well-trained male athletes completed 3 protocols in randomized order: 5 sets of fixed-repetition squats (Fixed), 5 sets of velocity-loss-threshold-controlled squats (VLT), and a control condition. Countermovement jump (CMJ) height was assessed at baseline, after each set, and at 8 post-training timepoints (5-60 minutes). Fatigue was quantified as the percentage of CMJ height relative to baseline, using both maximum and average values of 2 trials. Within-session reliability was evaluated via coefficient of variation (CV) and intraclass correlation coefficient (ICC).
Results:
For within-training assessment, significant main effects emerged for protocol (P = 0.006), value type (P = 0.031), and set number (P < 0.001), with lower values (greater fatigue) observed when using average values and in the final 2 sets. All protocols demonstrated acceptable within-session reliability (CV ≤ 5.29%, ICC ≥ 0.745). For post-training assessment, time point significantly affected fatigue (P < 0.001), with the lowest values at 30 to 40 minutes. ICC was low during 0 to 15 minutes post-training; acceptable reliability was achieved only with maximum values from 10 minutes onward (CV ≤ 7.29%, ICC ≥ 0.750).
Conclusion:
Both within-training and post-training fatigue assessments are feasible for monitoring resistance training fatigue, but methodological choices substantially influence measured values and reliability.
Clinical Relevance:
For within-training fatigue monitoring, average values provide greater sensitivity in detecting fatigue; for post-training assessment, maximum values should be used after ≥10 minutes of rest to ensure acceptable reliability.
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Exercise Stress Test
Exercise stress testing, commonly known as a treadmill test, is a noninvasive procedure used to evaluate cardiovascular function and diagnose heart conditions.
Definition
An exercise stress test measures the heart's response to exertion using a treadmill or stationary bicycle. Chest electrodes record the heart's electrical activity through an ECG, and blood pressure is monitored regularly.
Purposes
