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[Experience in validating equipment and reagents for immunohistochemical studies in the context of changing

O A Kuznetsova1, E M Olyushina1, L V Moskvina1,2

  • 1Russian Medical Academy of Continuous Professional Education, Moscow, Russia.

Arkhiv Patologii
|October 16, 2026
PubMed

Keywords:
devicesimmunohistochemical studiesstaining protocolsvalidation

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Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...