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Updated: Oct 18, 2026
Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
Harigabri-Derived Carbon Dots as FRET Probes for E. coli Analysis
Rile Ou1, Denggerile Ao1, Lu Ga2
1College of Chemistry and Environmental Science, Inner Mongolia Key Laboratory of Environmental Chemistry, Inner Mongolia Normal University, 81 zhaowudalu, Hohhot010022, China.
Abstract:
Green-fluorescent carbon quantum dots (CQDs) were hydrothermally synthesized from the Mongolian medicine Harigabri, exhibiting uniform size (∼2.5 nm) and strong emission at 521 nm under 468 nm excitation. Based on the fluorescence resonance energy transfer (FRET) between the CQDs (donors) and AgNPs (acceptors), an aptamer-functionalized biosensor (Apt-CQDs/AgNPs) was constructed for selective Escherichia coli detection. Target recognition by the aptamer inhibited FRET and restored fluorescence. The sensor showed a linear response from 9 × 102 to 9 × 107 CFU/mL with a detection limit of 5 CFU/mL. This work highlights Harigabri-derived CQDs as promising nanomaterials for rapid pathogen detection and demonstrates the potential of traditional medicinal resources in biosensing applications.
