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Updated: Oct 18, 2026

Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis
Published on: December 16, 2016
Assessing the global capacity for monitoring microplastics (0.3-5 mm) on sandy beaches: Insights from the first
Marc Metian1, Kristof Moeller1, Sabine Azemard1
1International Atomic Energy Agency (IAEA) Marine Environment Laboratories, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Monaco, 98000, Principality of Monaco.
Abstract:
Monitoring microplastics (MPs) has become a global priority requiring equitable analytical capacity, particularly in the Global South. Through the NUTEC Plastics initiative, the IAEA has addressed this need by developing a harmonized protocol and providing training and equipment to its Member States. The initial phase targeted large MPs (0.3-5 mm) in beach sand. To assess data comparability and analytical performance, a proficiency test was organized in which 49 laboratories from 41 countries analyzed a blind beach-sand sample spiked with a defined number and composition of MPs, reporting counts and polymer identity by size fraction. Recovery was strongly size-dependent. Performance was reliable for the large fraction (1-5 mm), where 22 laboratories (~45%) recovered the exact spiked count, in contrast to one laboratory (~2%) for the small fraction (0.3-1 mm). Of the 41 laboratories identifying polymers, 18 (~44%) failed to detect any transparent polystyrene particles. Generalized linear modelling identified the agitation method during density separation as the only significant predictor of polystyrene recovery, with mechanical shaking yielding ~1.77-fold higher recovery than magnetic stirring. Approximately one in four reported particles could not be attributed to spiked polymers. These findings identify targets and define priorities for the next capacity-building phase: improving recovery of small and transparent particles, and further standardizing agitation, contamination control, and data reporting. Altogether, this study provides a framework for method refinement and a step toward standardized global MP monitoring.
