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Updated: Oct 19, 2026
Diffusion Imaging in the Rat Cervical Spinal Cord
Published on: April 7, 2015
Accelerated Motion-Compensated Diffusion-Weighted pCASL Using Subspace Low-Rank Reconstruction for Quantification of
Zixuan Liu1,2, Chenyang Zhao1, Ziyang Huang1,2
1Laboratory of FMRI Technology (LOFT), Mark & Mary Stevens Neuroimaging and Informatics Institute, Keck School of Medicine, University of Southern California, Los Angeles, California, USA.
Purpose:
To develop an accelerated motion-compensated diffusion-weighted pseudo-continuous arterial spin labeling (MCDW-pCASL) method using a spatial subspace low-rank reconstruction method for efficient quantification of blood-brain barrier (BBB) water exchange (kw) and permeability (PSw).
Methods:
An accelerated multidelay MCDW-pCASL sequence was developed to simultaneously encode intravascular and extravascular diffusion-weighted ASL signals across multiple post-labeling delays (PLDs). A spatial subspace low-rank reconstruction framework was optimized to enable joint estimation of cerebral blood flow (CBF) and BBB water exchange rate and permeability. Fourteen young healthy adults underwent test-retest scans (separated by ∼1 week) at 3 T with both the accelerated MCDW-pCASL (acceleration factor = 3) and a conventional diffusion-prepared (DP) pCASL sequence. Whole-brain, gray-matter, and white-matter CBF and kw values were quantified to assess test-retest repeatability and cross-method agreement. An additional cohort of 30 older adults underwent single-session MCDW and DP scans to evaluate age-related perfusion and BBB kw/PSw differences. Intraclass correlation coefficients (ICCs) were used to assess reliability and agreement.
Results:
Accelerated MCDW-pCASL demonstrated excellent agreement with DP-pCASL for CBF (ICC = 0.89) and fair agreement for kw (ICC = 0.56). Test-retest repeatability of MCDW-pCASL was good for CBF, BBB, kw, and PSw (ICC ≈ 0.6). Across both sequences, younger subjects exhibited significantly higher CBF and kw compared with older adults.
Conclusion:
Incorporating a spatial low-rank subspace reconstruction enables accelerated MCDW-pCASL acquisition with reliable simultaneous quantification of CBF, BBB kw, and PSw. Clinical applications of this method for assessing perfusion and BBB function are warranted.
