Identification of Bioactive Compounds Produced by Probiotic Bacillus coagulans Using Gas Chromatography-Mass...

Metabolites from probiotic bacteria are gaining wider recognition as bioactive compounds having possible applications in the food, pharmaceutical, and healthcare sectors. Of these, Bacillus coagulans is one of the spore-producing probiotics recognized for its robustness and physiological benefits; however, there is a lack of information regarding the chemical diversity of these bacteria. This study aims to identify the bioactive compounds produced by B. coagulans (MTCC 9872) through the process of gas chromatography-mass spectrometry (GC-MS). Ethyl acetate, benzene, chloroform, hexane, and methanol were used to obtain the extracts of 4-day-old cultures. A total of 32 metabolites have been identified from diverse classes of chemicals including organic acids, hydrocarbons, carbohydrates, amino acid derivatives, phenols, and esters. Glycerol, azelaic acid, deoxyspergualin, and 2,4-di-tert-butylphenol are some of the important chemicals with antimicrobial, antioxidative, anti-inflammatory, immunomodulating, and antitumor activities. The current findings confirm that B. coagulans has potential applications in producing functional foods, nutraceuticals, and therapeutic products.