Related Experiment Video
Updated: Oct 20, 2026
Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
Published on: February 1, 2022
An acetylcholinesterase electrochemical biosensor based on a three-dimensional nanoporous PdCe nanocubes/graphene
Chunmei Gao1, Chengzong Zhu1, Hongyu Dai1
1Jiangsu Laboratory of Advanced Functional Materials, Department of Materials Engineering, Suzhou University of Technology, Changshu 215500, People's Republic of China. crj@szut.edu.cn.
Abstract:
Three-dimensional nanoporous PdCe (NP-PdCe) nanocubes were fabricated by dealloying PdCeAl alloy ribbons and integrated with graphene and chitosan to construct an acetylcholinesterase (AChE)-based electrochemical biosensor for malathion determination. The NP-PdCe nanocubes possess a bicontinuous nanoporous framework, mesoporous channels, and exposed Pd-Ce interfacial sites that provide an accessible surface for enzyme loading and promote thiocholine oxidation. Graphene (GR) forms a conductive network for electron transport, whereas chitosan provides a biocompatible film-forming matrix for enzyme immobilization. Under optimized conditions, the AChE/NP-PdCe/GR-CS/GCE biosensor exhibited two linear ranges (2.0-60 and 60-350 ng mL-1) and a reported detection limit of 1.30 ng mL-1. The sensor showed acceptable repeatability (RSD 3.35%), fabrication reproducibility (RSD 1.72%), four-week response retention (89.45%), and resistance to selected non-pesticide interferents. In practice, the biosensor was employed to detect malathion in tap water and cabbage samples with satisfactory recovery.
