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Updated: Oct 22, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
Single-axis scanning coherent diffractive imaging for spectroscopic and wavefront retrieval
Qijun You1, Lingshuo Meng1, Fangrui Quan1
1School of Physics and Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China.
Abstract:
We present single-axis scanning coherent diffractive imaging (AS-CDI), in which the detector remains fixed while the sample is translated along the optical axis through a spatially evolving probe. The axial evolution of the illumination provides additional redundancy for wavelength-resolved joint reconstruction of the object and the probe from a single scan. We validate the concept by simultaneous spectroscopic imaging and quantitative characterization of the illumination wavefront, from which the chromatic aberration of a broadband field is accurately measured. This capability establishes AS-CDI as a versatile tool for multi-modal imaging and reference-free wavefront and dispersion metrology, with immediate potential in ultrafast science, material characterization, and live-cell bio-imaging.
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