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Published on: June 25, 2020
OsSHR2 and OsHB1 modulate phosphorus accumulation in rice
Zhi Hu1, Xu Huang2, Zhantian Zhang3
1State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China; School of Agriculture and Biotechnology, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, Guangdong, China.
Abstract:
Plants acquire inorganic phosphate (Pi) through specialized phosphate transporters, whose expression is transcriptionally regulated by transcription factors. SHORTROOT (SHR) and its downstream transcription factors gene PHABULOSA (PHB) play critical roles in plant development; however, their involvement in the rice P regulatory network remains unclear. This study investigated the roles of OsSHR2 and its downstream gene OsHB1(OsHOMEOBOX1) in rice P accumulation. OsSHR2 encodes a transcriptionally active protein, and its expression is induced under Pi deficiency. Knockout of OsSHR2 exhibited enhanced P accumulation under high phosphate conditions, but impaired rice yield per plant due to reduced 1000-grain weight and seed setting rate. Correspondingly, knockout of OsHB1-which is negatively regulated by OsSHR2-showed reduced Pi accumulation and impaired rice yield under Pi-deficient conditions. Multiple experimental approaches demonstrated that OsHB1 binds to the promoter of PHOSPHATE STARVATION RESPONSE2 (OsPHR2) to activate its expression, thereby regulating P accumulation. Collectively, our results demonstrate that OsSHR2 and OsHB1 regulate P accumulation, uncovering a novel regulatory pathway for Pi homeostasis in rice.
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