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Updated: Oct 24, 2026
Rapid Detection of Fecal Antigen of Helicobacter pylori Infection Based on Double Antibody Sandwich Detection Technology
Published on: May 23, 2025
Comparison of two chemiluminescence assays for the detection of Helicobacter pylori antigen in stool samples
Alba García-Sáez1, Blanca Carrasco1, Óscar Cuevas1
1Servicio de Microbiología, Hospital Universitario de Getafe, Getafe, Madrid, Spain.
Introduction:
Non-invasive methods, particularly stool antigen detection, are widely employed for the diagnosis of Helicobacter pylori infection, and the evaluation of new diagnostic platforms is of practical interest. We evaluated the agreement between two chemiluminescence techniques, CLIA LIAISON® H. pylori SA and the recently developed H. pylori Ag VIRCLIA® Monotest.
Methods:
We analysed 200 stool samples from patients with suspected infection.
Results:
92 samples were positive and 94 negative in both tests. Of the discordant samples, 9 were positive by LIAISON® and negative by VIRCLIA®, and 5 were positive by VIRCLIA® and negative by LIAISON®. Overall agreement was 93.0%, positive percent agreement (PPA) was 92.9%, and negative percent agreement (NPA) was 93.1%. Kappa coefficient was 0.86 (95% CI 0.79-0.93; p<0.0001).
Conclusions:
The VIRCLIA® assay demonstrated a high level of agreement with the LIAISON® assay for the detection of H. pylori stool antigen.
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