Protein-Centric Biochemistry Laboratory Experiments from Protein Quantification to Proteomics

This series of experiments for the Biochemistry and Biotechnology Laboratory is designed to provide students with a comprehensive introduction to fundamental biochemistry laboratory techniques. Throughout the experiments, students gain hands-on experience in protein quantification, protein separation and purification, and protein characterization using spectroscopic and electrophoretic methods. The workflow progresses from basic analytical techniques to more advanced approaches, including chromatographic separations, gel electrophoresis, western blotting, enzyme isolation and kinetics, and finally mass spectrometry-based protein analysis.