Quantifying Data Reuse in Proteomics Using PRIDE Downloads Statistics and a Semi-Supervised LLM-Based Framework

Understanding how scientific datasets are accessed and reused is essential for resource planning and impact assessment. Here we present the PRIDE Archive download tracking infrastructure and a comprehensive analysis of 159.3 million download records from the PRIDE proteomics database (2021-2025), spanning 35,528 datasets accessed from 235 locations. The infrastructure includes nf-downloadstats, a scalable Nextflow pipeline for processing download logs, and DeepLogBot, a machine-learning framework that classifies traffic into bots, institutional download hubs, and independent user downloads. DeepLogBot combines heuristic seed selection with multi-LLM annotation (Claude and Qwen3) to produce LLM-assisted consensus labels, achieving 92.2% bot classification accuracy on a held-out test set. After separating bot traffic, analysis reveals downloads from 214 countries/regions, 249 institutional download hubs, and a concentrated reuse distribution, with the top five countries (United States, United Kingdom, Germany, China, and Canada) accounting for over 54% of independent user downloads. These findings provide actionable insights for repository infrastructure planning and highlight the importance of distinguishing automated from individual access in scientific data resources.