Protocol for Cryopreservation of Bacillus Strains with Biotechnological Potential

Cryopreservation is an important method in microbiology that guarantees the genetic stability and long-term viability of microbial strains of biotechnological significance, including heat-resistant bacteria of the Bacillus genus. The most prevalent and efficient method for bacteria is the use of glycerol as a cryoprotective agent at low temperatures, typically -80°C. This protocol delineates a technique for preserving three thermoresistant strains (B. licheniformis M2-7, B. subtilis ANF2, and B. tequilensis M2) with great biotechnological potential using glycerol. This is a low-cost, dependable, and standard method for preserving the integrity of bacterial strains in research laboratories, thereby increasing their utility for future investigations.