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Updated: Oct 25, 2026

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
Published on: June 25, 2020
Phosphorylation-Mediated Regulatory Networks Fine-Tune Rye Responses to PVC Nanoplastic Stress: Evidence from
Jinke Hu1, Guozhang Bao1, Baiming Guo1
1Key Laboratory of Groundwater Resources and Environment of the Ministry of Education (Jilin University); Jilin Provincial Key Laboratory of Water Resources and Environment; College of New Energy and Environment, Jilin University, Changchun, 130012, China.
Abstract:
Although polyvinyl chloride nanoplastics (NPs-PVC) are widespread emerging contaminants, their crop toxicity mechanisms─especially at the post-translational modification (PTM) level─remain unclear. Using multiscale exposures (short-term hydroponics and whole-life-cycle soil cultivation), integrated physiological, imaging, omics (transcriptomics and phosphoproteomics), molecular docking, and molecular dynamics simulations, we systematically evaluated NPs-PVC impacts on Secale cereale. Short-term exposure inhibited growth, reduced photosynthesis, and induced oxidative stress, with PVC stably binding cytochrome f and catalase. Long-term exposure decreased grain weight and tillers, accompanied by transcriptional shifts in metabolism and stress pathways. Phosphoproteomics revealed widespread phosphorylation changes in photosynthesis- and lipid metabolism-related proteins, with PVC-14-3-3λ binding confirmed by simulation. Structural equation modeling demonstrated that NPs-PVC regulates agronomic traits mainly through oxidative stress and photosynthesis disruption. Collectively, NPs-PVC suppresses rye growth via direct protein binding, transcriptional reprogramming, and PTM-mediated physiological tuning. This study advances the understanding of nanoplastic phytotoxicity mechanisms and agricultural ecological risk assessment.
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